Wednesday, February 5, 2014


VIRTUAL MICROSCOPE LAB
Part 1. Fill in these blanks and answer questions from information in the Introductory Video. Retype question and answer into your blog.
    •    When you begin, the condenser knob should be all the way up.
    •    Start with the 4x objective in place.
    •    Raise the rheostat to 10.
    •    Move slide using the X Y controls.
    •    Why do you close the iris before looking into the microscope?  You close the iris before looking into the microscope so that when you look through the oculars, you don't blind yourself.
    •    Eyes should be about how far from oculars?  Your eyes should be approximately 3/4 inch away from the oculars.
    •    Increase light by opening the iris.
    •    Highest magnifying objective is called oil immersion.
    •    Describe the oil immersion procedure.  To place a drop of oil on the slide, you are going to move the objective so specimen between 140x objective, then place one drop of oil right over the specimen, you may want to adjust light. 
    •    Clean slides with lower stage and remove slide then wipe with a kimwipe.  Clean objectives facing out with a piece of lens paper.
    •    What is the procedure for shutting down the microscope?   In order to shut down the microscope, there are procedures that are important.  First make sure 4x objective is in place,  close iris, turn rheostat down to 1, then turn off microscope. 
    •   
Part 2:
  1. What objective must be in place when you remove slide or place a new slide on the stage of the scope?  A 4X objective should be in place when you remove slide or place a new slide on the stage of the scope.
  2. What objective must always be used when you first start looking at a slide?  You should use the 4X objective when you first start looking at your slide.
  3. You must adjust the amount of light when looking at a thin and or transparent cells or tissue section such as onion or amoeba cells.
  4. Describe two ways you can decrease light intensity, the amount of light passing though the scope.  You can decrease the light intensity by adjusting the iris and also by setting the rheostat to 1.
  5. Which objective allows you to see the larges area of the object that you are viewing?  The 4X objective allows you to view the largest area of the slide.
  6. How much are you magnifying something when you are using 10x oculars and 10x objectives?  When you are using 10x oculars and 10x objectives, you are magnifying something by 100x.
  7. How much are you magnifying something when you are using 10x oculars and 4x objectives?  When you are using 10x oculars and 4x objectives, you are magnifying something by 40x.
  8. Which focusing knobs/adjustments do you first use when you begin to looking at a slide?  The knob you first use when you begin to look at a slide is the course focus knob.
  9. What do you adjust if you can see two overlapping circles with part of the object in each circle?  To adjust two overlapping circles, you need to adjust the oculars.
Part 3:
1)      View bacterial capsule, cheek smear, letter e, and onion root.  CHECK
2)      Take a photo of two slides as they appear while looking through the virtual microscope while on “You are looking through the microscope” view (one photo of two slides). Add the photo to your blog post.
Letter E:

Bacterial Capsule:
















3)      Describe the appearance of each of the object(s) as they appear while looking through the virtual microscope.
Letter E on objective 4x looks like a regular, upside down, letter "e", but when using objective 10x, the letter starts to look as if it has some sort of cell membrane, but it's just pixilated.
Bacterial Capsule: You can tell the capsule has a nucleus and a shell.  Also it's noticeable the bacteria has neutrons.
4)      Name one visible structure on each of the organism slides.
Letter E:  This "organism" is a solid object.
Bacterial Capsule: This organism has a nucleus.
Onion Root: This organism has many neutrons.
Cheek Smear: This organism does not have a shell. 

2 comments:

  1. Wow. This looks great. Thanks for a nicely organized blog post.

    ReplyDelete
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